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Image Search Results
Journal: The Journal of Clinical Investigation
Article Title: Disruption of PPAR?/?-catenin-mediated regulation of apelin impairs BMP-induced mouse and human pulmonary arterial EC survival
doi: 10.1172/JCI43382
Figure Lengend Snippet: (A) Caspase 3/7 activity was used to measure the level of apoptosis in PAECs exposed for 24 hours to SF medium in the presence of vehicle control, apelin (100 nM), or VEGFA (50 ng/ml). (B) PAEC survival under these conditions was also determined by cell count and MTT analyses. (C) Survival in the presence of apelin was also assessed by cell counts in PMVECs isolated from control and IPAH patients. (D) Apoptosis level in control siRNA– and apelin siRNA–treated PAECs, as determined by caspase 3/7 assay under SF conditions. (E) Survival of apelin-deficient PAECs was determined by cell count after 24 hours of exposure to SF conditions. Baseline represents cell number before switching to SF medium. Bars represent mean ± SEM from 3 separate experiments with 6 replicates per condition in A and D and 3 replicates per condition in B, C, and E. *P < 0.05, **P < 0.01, ***P < 0.001 vs. respective control, 1-way ANOVA with Bonferroni multiple comparison test.
Article Snippet:
Techniques: Activity Assay, Control, Cell Counting, Isolation, Comparison